2024
An integrated multi-omics approach reveals polymethoxylated flavonoid biosynthesis in Citrus reticulata cv. Chachiensis
Jiawen Wen*, Yayu Wang*, Xu Lu*, Huimin Pan*, Dian Jin, Jialing Wen, Canzhi Jin, Sunil Kumar Sahu, Jianmu Su, Xinyue Luo, Xiaohuan Jin, Jiao Zhao, Hong Wu#, E-hu Liu#, Huan Liu# (* equal contribution, # corresponding author)
Nature Communications 2024 Spotlight
A key enzyme involved in polymethoxylated flavonoid biosynthesis was identified through an integrated multi-omics approach.
An integrated multi-omics approach reveals polymethoxylated flavonoid biosynthesis in Citrus reticulata cv. Chachiensis
Jiawen Wen*, Yayu Wang*, Xu Lu*, Huimin Pan*, Dian Jin, Jialing Wen, Canzhi Jin, Sunil Kumar Sahu, Jianmu Su, Xinyue Luo, Xiaohuan Jin, Jiao Zhao, Hong Wu#, E-hu Liu#, Huan Liu# (* equal contribution, # corresponding author)
Nature Communications 2024 Spotlight
A key enzyme involved in polymethoxylated flavonoid biosynthesis was identified through an integrated multi-omics approach.
2023
Host Genetic Traits Underlying the Composition and Assembly of the Citrus Microbiome
Jin Xu*, Yayu Wang*, Yunzeng Zhang, Nadia Riera, Jinyun Li, Kelly J. Clark, Tao Jin, Hongyun Chen, Jiawen Wen, Wenbo Ma, Huan Liu#, Nian Wang# (* equal contribution, # corresponding author)
Phytobiomes Journal 2023
Through genome-wide association study analysis of 66 citrus rootstock-scion combinations and their corresponding leaf, root, and rhizosphere metagenomes, we identified host genetic determinants associated with microbiome composition and function, revealing that plant genes involved in immunity, transport, hormones, and metabolism shape microbial communities, while bacterial colonization traits differ among plant compartments.
Host Genetic Traits Underlying the Composition and Assembly of the Citrus Microbiome
Jin Xu*, Yayu Wang*, Yunzeng Zhang, Nadia Riera, Jinyun Li, Kelly J. Clark, Tao Jin, Hongyun Chen, Jiawen Wen, Wenbo Ma, Huan Liu#, Nian Wang# (* equal contribution, # corresponding author)
Phytobiomes Journal 2023
Through genome-wide association study analysis of 66 citrus rootstock-scion combinations and their corresponding leaf, root, and rhizosphere metagenomes, we identified host genetic determinants associated with microbiome composition and function, revealing that plant genes involved in immunity, transport, hormones, and metabolism shape microbial communities, while bacterial colonization traits differ among plant compartments.
Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy
Xiaoen Huang*, Hongge Jia*, Jin Xu, Yuanchun Wang, Jiawen Wen, Nian Wang# (* equal contribution, # corresponding author)
Nature Plants 2023
We developed a co-editing strategy using transient expression of cytosine base editor, Cas12a/CRISPR RNA, and GFP to achieve transgene-free genome editing in T0 generation plants.
Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy
Xiaoen Huang*, Hongge Jia*, Jin Xu, Yuanchun Wang, Jiawen Wen, Nian Wang# (* equal contribution, # corresponding author)
Nature Plants 2023
We developed a co-editing strategy using transient expression of cytosine base editor, Cas12a/CRISPR RNA, and GFP to achieve transgene-free genome editing in T0 generation plants.
2022
Transcriptomic analyses provide new insights into green and purple color pigmentation in Rheum tanguticum medicinal plants
Haixia Chen*, Tsan-Yu Chiu*, Sunil Kumar Sahu, Haixi Sun, Jiawen Wen, Jianbo Sun, Qiyuan Li, Yangfan Tang, Hong Jin#, Huan Liu# (* equal contribution, # corresponding author)
PeerJ 2022
Through multi-tissue transcriptomic analysis of Rheum tanguticum, we discovered that high expression of chlorophyll degradation enzymes (RtPPH, RtPao, and RtRCCR) in purple leaf samples contributes to their distinctive coloration, providing valuable insights into the genetic basis of pigmentation in this important medicinal plant.
Transcriptomic analyses provide new insights into green and purple color pigmentation in Rheum tanguticum medicinal plants
Haixia Chen*, Tsan-Yu Chiu*, Sunil Kumar Sahu, Haixi Sun, Jiawen Wen, Jianbo Sun, Qiyuan Li, Yangfan Tang, Hong Jin#, Huan Liu# (* equal contribution, # corresponding author)
PeerJ 2022
Through multi-tissue transcriptomic analysis of Rheum tanguticum, we discovered that high expression of chlorophyll degradation enzymes (RtPPH, RtPao, and RtRCCR) in purple leaf samples contributes to their distinctive coloration, providing valuable insights into the genetic basis of pigmentation in this important medicinal plant.
GWAS, MWAS and mGWAS provide insights into precision agriculture based on genotype-dependent microbial effects in foxtail millet
Yayu Wang*, Xiaolin Wang*, Shuai Sun, Canzhi Jin, Jianmu Su, Jinpu Wei, Xinyue Luo, Jiawen Wen, Tong Wei, Sunil Kumar Sahu, Hongfeng Zou, Hongyun Chen, Zhixin Mu, Gengyun Zhang, Xin Liu, Xun Xu, Lone Gram, Huangming Yang, Ertao Wang#, Huan Liu# (* equal contribution, # corresponding author)
Nature Communications 2022
Through integrated GWAS, MWAS, and mGWAS analysis of 827 foxtail millet cultivars, we identified 257 rhizoplane microbial biomarkers associated with agronomic traits and revealed that plant genes controlling immunity, metabolism, and nutrient uptake shape microbiota composition, which in turn influences plant growth in a genotype-dependent manner.
GWAS, MWAS and mGWAS provide insights into precision agriculture based on genotype-dependent microbial effects in foxtail millet
Yayu Wang*, Xiaolin Wang*, Shuai Sun, Canzhi Jin, Jianmu Su, Jinpu Wei, Xinyue Luo, Jiawen Wen, Tong Wei, Sunil Kumar Sahu, Hongfeng Zou, Hongyun Chen, Zhixin Mu, Gengyun Zhang, Xin Liu, Xun Xu, Lone Gram, Huangming Yang, Ertao Wang#, Huan Liu# (* equal contribution, # corresponding author)
Nature Communications 2022
Through integrated GWAS, MWAS, and mGWAS analysis of 827 foxtail millet cultivars, we identified 257 rhizoplane microbial biomarkers associated with agronomic traits and revealed that plant genes controlling immunity, metabolism, and nutrient uptake shape microbiota composition, which in turn influences plant growth in a genotype-dependent manner.